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KMID : 0604520190450040333
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Journal of the Society of Cosmetic Scientists of Korea
2019 Volume.45 No. 4 p.333 ~ p.340
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Analytical Method Development of Avicularin and Quercitrin in Astilbe chinensis Extract Using HPLC
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Kwon Jin-Gwan
Jung Yeon-Woo
Seo Chan-Gon
Hong Seong-Su
Shin Hyun-Tak
Jung Su-Young
Choi Jeong-June
Kim Jin-Kyu
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Abstract
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This study attempted to eatablish a high performance liquid chromatography (HPLC) analysis method for the determination of avicularin, quercitrin as a part of the quality control for the development of functional cosmetic materials from Astilbe chinensis extract. HPLC was performed on a Capcell Pak C18 MGII column with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and acetonitrile at a flow rate of 1.0 mL/min at 30 ℃. The analyte was detected at 254 nm. The HPLC method was performed in accordance with the international conference on harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation of avicularin and quercitrin were 0.094 and 0.285 mg/mL, 0.031 and 0.095 mg/mL respectively. Calibration curves showed good linearity r2 > 0.99990 for avicularin and r2 > 0.99994 for quercitrin. Precision of analysis was satisfied with less than 0.59% for avicularin and 0.63% for quercitrin. Recoveries of quantified compounds ranged from 100.97 to 101.77% for avicularin and 100.18 to 100.32% for quercitrin. These result indicated that the established HPLC method is very useful for the determination of marker compounds in A.
chinensis extracts.
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KEYWORD
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Astilbe chinensis, avicularin, functional cosmetic, HPLC, quercitrin, validation
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